Evaluation of amplified ribosomal DNA restriction analysis (ARDRA) and species-specific PCR for identification of Bifidobacterium species

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Authors	KŘÍŽOVÁ Jana ŠPANOVÁ Alena RITTICH Bohuslav
Year of publication	2006
Type	Article in Periodical
Magazine / Source	Systematic and Applied Microbiology
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	Bifidobacterium spp.; identification; PCR; ARDRA
Description	Molecular biological methods based on genus and species specific PCR and restriction analysis of two PCR amplicons using six restriction enzymes were used to differentiate among species of Bifidobacterium. Optimalised methods were applied for identification of strains collected in Culture Collection of Dairy Microorganisms. Restriction analysis of longer PCR amplicon was the most discriminative for Bifidobacterium species differentiation. Practicaly observed restriction profiles were compared and confirmed with the theoretical restriction analysis of Bifidobacterium sequences obtained from GenBank database.
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