Genetic mapping and DNA markers for gene identification in Arabidopsis thaliana
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005 |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Arabidopsis thaliana; CAPS; DNA markers; genetic mapping; microsatellites |
| Description | During the last fifteen years the efforts of geneticists and molecular biologists have been concentrated on the study and understanding of the molecular basis of plant development and identification and characterisation of new genes. In this field Arabidopsis thaliana (L.) Heynh. has played an important role as the major plant model system. It is the first plant species whose all genome was sequenced (AGI, 2000) but our knowledge of every gene function is low so far. Mutagenesis and molecular-genetic strategies have been developed to facilitate the isolation of new genes and became an attractive method for functional analysis (MEINKE et al., 2003). The main goal of our study was gene identification and localization on the genetic map of A. thaliana. We have been working with both classical (ethyl methanesulfonate, EMS) and insertional (T-DNA) collections of mutants. Altogether 10 genes were assigned on genetic map by recombination analysis of F2 plants. The known location of genes on genetic map will facilitate the decision of eventual allelism with mutants already isolated by previous authors. |
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