Degradation of 5-nitroguaiacol by soil bacteria of the genus Rhodococcus

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Authors

NAVRÁTILOVÁ Jitka TVRZOVÁ Ludmila NEČA Jiří NĚMEC Miroslav

Year of publication 2004
Type Article in Periodical
Magazine / Source Folia Microbiologica
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords 5-nitroguaiacol; Rhodococcus; degradation
Description The bacterial strains J2 and J3 were isolated from forest soil by selective enrichment of the mineral medium containing 4-nitrocatechol as the sole carbon and energy source. Both strains could utilize 4-nitrocatechol and 5-nitroguaiacol as sole carbon and energy sources. Degradation of 5-nitroguaiacol and stechiometric release of nitrites was measured during degradation of 5-nitroguaiacol both in growth culture and for resting cells. Both strains were unable to degrade 4-nitroguaiacol, 2-nitrophenol, 3-nitrophenol, 4-nitrophenol, 2,4-dinitrobenzoic acid, 4,5-dimethoxy-2-nitrobenzoic acid and 2,3-difluoro-6-nitrophenol. The strain J2 was identified as Rhodococcus opacus and the strain J3 as Rhodococcus sp. The J3 strain is deposited in the Czech Collection of Microorganisms as Rhodococcus sp. CCM 4930.
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