Identification of human Bifidobacterium strains using genus and species specific PCR

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Authors	RITTICH Bohuslav ŠPANOVÁ Alena DRÁB Vladimír
Year of publication	2005
Type	Article in Proceedings
Conference	8th Symposium on Lactic Acid Bacteria
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	Bifidobacterium human strains; PCR; ARDRA; fermentation profiles
Description	In the present study genus-specific and species-specific PCR primers were used to identify Bifidobacterium strains isolated from infant faecal samples. Bifidobacteria were differentiated at the species level by a restriction using the enzymes BamHI, Sau3AI, and TaqI (amplified ribosomal DNA restriction analysis ARDRA). Altogether 60 strains of Bifidobacteria identified in the collected infant faeces were analysed. Selected bifidobacteria were also differentiated using REP-PCR and RAPD analysis. Fermentation profiles and some technological characteristics of selected bacterial strains, as a surviving at low pH, resistance to bile salts, and the influence of isolated strains on the growth of selected starter cultures were also studied.
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