Genomová amplifikace a restrikční mapování patogenních spirochet rodu Treponema.
| Title in English | Genomic amolifications and restriction mapping of pathogenic spirochetes of the genus Treponema. |
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| Authors | |
| Year of publication | 2005 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Genomes of closely related treponemes, Treponema pallidum ssp. pallidum (strains Nichols and SS14) and Treponema pallidum ssp. pertenue (Gauthier, Samoan D) were compared with the genome of Treponema paraluiscuniculi using genomic techniques. Whole genome fingerprinting was used to study treponemal genomes. PCR-amplified regions of genomic DNA were digested with BamH I, EcoR I, Hind III, or their combinations. PCR amplification and subsequent restriction analysis of the complete genomes of the strains Nichols, SS14 and Gauthier revealed 1 absent restriction site in SS14 and Gauthier. When compared to Nichols genome, 8 and 34 differences in the target restriction sites and 1 and 8 detectable deletions/insertions were found in the genome of Samoan D and T. paraluiscuniculi, respectively. |
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