Restrikční mapování genomů blízce příbuzných spirochet rodu Treponema.
| Title in English | restriction mapping of the treponemal genomes |
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| Authors | |
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | Chemické listy |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Treponema pallidum ssp. pallidum; Treponema pallidum ssp. pertenue; Treponema paraluiscuniculi |
| Description | Restriction mapping was used for comparison of four treponemal genomes including strains T. pallidum subsp. pallidum Nichols, T. pallidum subsp. pallidum SS14, T. pallidum subsp. pertenue Samoan D and T. paraluiscuniculi. The genomes of analysed strains were divided into 94 intervals (TPI intervals) covering the whole genome and each of these segments was amplified by XL PCR. These PCR products were digested by restriction enzymes BamH I, EcoR I and Hind III and the resulting restiction profiles were used for identification of heterologous regions in chromosomal DNA. The results indicate a high sequence homology among treponemal strains. Different degrees of virulence of treponemal strains thus appear to depend on relatively small differences in their genomes. The genome differences are preferentially localized in tpr genes. Identified nucleotide changes can be used for molecular identification of individual treponemal strains or isolates. |
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