Development of Arabidopsis and Tobacco Seedling Shoot Apical Meristem after ipt Transcriptional Activation in pOp/LhG4 System
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | Acta Biologica Cracoviensia, ser. Bot. 47 (Suppl.1), Abstracts XII International Conference on Plant Embryology |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Cytokinins; ipt activation; meristem development |
| Description | A transcription activation system pOp::ipt/CaMV35S::LhG4 was used in tobacco and Arabidopsis for the study of ipt (a cytokinin biosynthesis gene) expression and its impact on post-embryonic development. Shoot apical meristem development in seedlings of both wild type and all transgenic lines aseptically grown on modified MS medium was studied by means of classical histological methods. Phenotypic changes typical for cytokinin overproduction were clearly observable on plantlets with activated ipt expression after 7 days of culture and they were more pronounced later. The shift of developmental stages to more progressed flower formation was traced in ipt-activated lines. |
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