Simultaneous HPLC determination of markers of metabolic activity of CYP1A2 and CYP2C6

• Authors: JUŘICA Jan; TOMANDL Josef; ZAHRADNÍKOVÁ Lucia; ZENDULKA Ondřej; HADAŠOVÁ Eva
• Year of publication: 2006
• Type: Article in Proceedings
• Conference: Book of abstracts, Central/Eastern European CINP Regional meeting
• MU Faculty or unit: Faculty of Medicine
• Field: Pharmacology and pharmaceutical chemistry
• Keywords: determination; cytochrome; CYP2C6; CYP1A2
• Description: The presented method allows to determine concentration of both markers of metabolic activity of CYP1A and CYP2C (phenacetine and tolbutamide) and their metabolites paracetamol and 4-hydroxytolbutamide in rat liver perfusate, respectively. This method is suitable for modeling and description of possible pharmacological interactions on isoforms CYP1A2 and CYP2C6 in rat.

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