Low-frequency magnetic field effect on cytoskeleton and chromatin

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Authors	KROUPOVÁ Jana BÁRTOVÁ Eva FOJT Lukáš STRAŠÁK Luděk KOZUBEK Stanislav VETTERL Vladimír
Year of publication	2007
Type	Article in Periodical
Magazine / Source	Bioelectrochemistry
MU Faculty or unit	Faculty of Medicine
Citation
Field	Biophysics
Keywords	ELF; low-frequency magnetic field; cytosceleton; chromatin; apoptosis;
Description	We studied low-frequency magnetic field effect on cytoskeleton and on the structure of chromatin in human cells. We used cell line of small lung carcinoma (A549) and the effects of magnetic field on cytoskeleton and higher-order chromatin structure were analyzed 96 h of magnetic field exposure. Magnetic field induction was Bm = 2 mT and the net frequency f = 50 Hz. In such affected and control cells the F-actin was estimated using FITC-conjugated Phalloidin and mitochondria were studied using MitoTracker (Molecular Probes). Images of cytoskeleton and genetic loci were acquired using confocal microscopy and analysis was performed by FISH 2.0 software. Slight morphological changes of F-actin filaments and mitochondria were observed in affected cells and nuclear condensation was found.
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