Determination of asymmetric dimethylarginine in human plasma by HPLC with coulometric detection
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | HPLC 2007. Abstract Book. |
| MU Faculty or unit | |
| Citation | |
| Web | 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques - HPLC 2007 |
| Field | Analytic chemistry |
| Keywords | asymmetric dimethylarginine; HPLC; coularray detection |
| Description | Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. ADMA is a competitive inhibitor of nitric oxide synthase. We report here development of a new HPLC method for the determination of ADMA using coulometric detection. After solid-phase extraction procedure on a strong cation exchange column and evaporation of the eluate under gentle stream of nitrogen at 70 to 75 C the reconstituted samples were derivatised with ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a reversed phase column Hydro-RP using isocratic elution and coulometric detection. |
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