Identification of powdery mildew resistance genes in a new accession of Hordeum vulgare ssp. spontaneum using DNA markers
| Authors | |
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| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | Cereal science and technology for feeding ten billion people: genomics era and beyond |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Hordeum vulgare; barley; powdery mildew; DNA markers; resistance |
| Description | An accession of wild barley (Hordeum vulgare ssp. spontaneum) carrying a newly identified resistance to powdery mildew (Blumeria graminis f. sp. hordei) was studied to localize the resistance genes on barley genetic map. Both genetic and molecular analyses were performed in the F2 population of the cross between the winter barley cultivar Tiffany and the resistant accession PI466197. The genetic analysis showed that the resistance is determined by two independent dominant genes and that one of the genes is in or in very close linkage with the Mla locus. DNA markers from on-line databases and literature were used for the localization of resistance genes. The susceptible F2 plants were screened to identify the markers linked with the resistance genes. Data from F2 plants of all genotypes were analysed in the MapManager QTX program to confirm the linkage and to assign the order and distances between the markers. The molecular analysis revealed a highly significant linkage with the markers Bmac0213 and MGB402 on chromosome 1H and Bmac0134, MWG878 and cMWG682 on chromosome 2H. The prospect of the work is to find more tightly linked DNA markers so that breeders could use them for marker assisted selection. |
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