Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | Book of Abstracts |
| MU Faculty or unit | |
| Citation | |
| Web | Advances in chromatography and electrophoresis 2007 & Chiranal 2007 |
| Field | Analytic chemistry |
| Keywords | assymetric dimethylarginine; HPLC |
| Description | Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. We report here development of a new HPLC method for the determination of ADMA using fluorescent detection. After solid-phase extraction procedure and evaporation of the eluate under gentle stream of nitrogen the reconstituted samples were derivatised with an ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a Hydro-RP column using isocratic elution and fluorescence detection. |
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