Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface.
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | European Biophysics Journal |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | amalgam electrodes; oligodeoxynucleotides detection |
| Description | The application of a gold amalgam-alloy electrode (AuAE) for a sensitive voltammetric detection of different oligodeoxynucleotides (ODNs) containing the purine units within the ODN-chains in the presence of copper ions is described. Our result shows that the sensitive detection of different ODNs can be also performed at the platinum amalgam-alloy electrode (PtAE) contrary to a low sensitivity at the silver amalgam-alloy electrode (AgAE).Electrochemical impedance spectroscopy (EIS), voltammetry, AFM, and DOE was used for the investigations of adsorption of oligonucleotides on the titanium surface and the effect of mechanical (polished, etched and/or gritted surface) and chemical modification of titanium surface was studied. From the EIS, voltammetric and optical measurements the conclusions of the effect of solvent and physical or chemical modification of titanium surface on the purine (AAG)12 and pyrimidine (TTC)12 oligonucleotide adsorption was delivered. |
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