Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

Adewumi, O.; Aflatoonian, B.; Ahrlund-Richter, L.; Amit, M.; Andrews, P.W.; Beighton, G.; Bello, P.A.; Benvenisty, N.; Berry, L.S.; Bevan, S.; Blum, B.; Brooking, J.; Chen, K.G.; Choo, A.B.; Churchill, G.A.; Corbel, M.; Damjanov, I.; Draper, J.S.; Dvořák,  Petr; Emanuelsson, K.; Fleck, R.A.; Ford, A.; Gertow, K.; Gertsenstein, M.; Gokhale, P.J.; Hamilton, R.S.; Hampl,  Aleš; Healy, L.E.; Hovatta, O.; Hyllner, J.; Imreh, M.P.; Itskovitz-Eldor, J.; Jackson, J.; Johnson, J.L.; Jones, M.; Kee, K.; King, B.L.; Knowles, B.B.; Lako, M.; Lebrin, F.; Mallon, B.S.; Manning, D.; Mayshar, Y.; McKay, R.D.; Michalska, A.E.; Mikkola, M.; Mileikovsky, M.; Minger, S.L.; Moore, H.D.; Mummery, C.L.; Nagy, A.; Nakatsuji, N.; O´Brien, C.M.; Oh, S.K.; Olsson, C.; Otonkoski, T.; Park, K.Y.; Passier, R.; Patel, H.; Patel, M.; Pedersen, R.; Pera, M.F.; Piekarczyk, M.S.; Pera, R.A.; Reubinoff, B.E.; Robins, A.J.; Rossant, J.; Rugg-Hunn, P.; Schulz, T.C.; Semb, H.; Sherrer, E.S.; Siemen, H.; Stacey, G.N.; Stojkovic, M.; Suemori, H.; Szatkiewicz, J.; Turetsky, T.; Tuuri, T.; Van den Brink, S.; Vintersten, K.; Vuoristo, S.; Ward, D.; Weaver, T.A.; Young, L.A.; Zhang, W.

Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

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Authors	ADEWUMI O. AFLATOONIAN B. AHRLUND-RICHTER L. AMIT M. ANDREWS P.W. BEIGHTON G. BELLO P.A. BENVENISTY N. BERRY L.S. BEVAN S. BLUM B. BROOKING J. CHEN K.G. CHOO A.B. CHURCHILL G.A. CORBEL M. DAMJANOV I. DRAPER J.S. DVOŘÁK Petr EMANUELSSON K. FLECK R.A. FORD A. GERTOW K. GERTSENSTEIN M. GOKHALE P.J. HAMILTON R.S. HAMPL Aleš HEALY L.E. HOVATTA O. HYLLNER J. IMREH M.P. ITSKOVITZ-ELDOR J. JACKSON J. JOHNSON J.L. JONES M. KEE K. KING B.L. KNOWLES B.B. LAKO M. LEBRIN F. MALLON B.S. MANNING D. MAYSHAR Y. MCKAY R.D. MICHALSKA A.E. MIKKOLA M. MILEIKOVSKY M. MINGER S.L. MOORE H.D. MUMMERY C.L. NAGY A. NAKATSUJI N. O´BRIEN C.M. OH S.K. OLSSON C. OTONKOSKI T. PARK K.Y. PASSIER R. PATEL H. PATEL M. PEDERSEN R. PERA M.F. PIEKARCZYK M.S. PERA R.A. REUBINOFF B.E. ROBINS A.J. ROSSANT J. RUGG-HUNN P. SCHULZ T.C. SEMB H. SHERRER E.S. SIEMEN H. STACEY G.N. STOJKOVIC M. SUEMORI H. SZATKIEWICZ J. TURETSKY T. TUURI T. VAN DEN BRINK S. VINTERSTEN K. VUORISTO S. WARD D. WEAVER T.A. YOUNG L.A. ZHANG W.
Year of publication	2007
Type	Article in Periodical
Magazine / Source	Nature biotechnology
MU Faculty or unit	Faculty of Medicine
Citation
Field	Genetics and molecular biology
Keywords	human; embryonic; stem cell
Description	This study represents the result of the first international comparison of a large number of lines of human embryonic (ES) stem cells from laboratories worldwide that has been accomplished with the aim to reveal and characterize their similarities and/or differences. In this study, we have analyzed 59 independently derived human ES cell lines, from 17 laboratories in 11 countries. Our data show that despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibit similar expression patterns for several markers of human ES cells. Nevertheless, the lines were not identical: differences in expression of several lineage-specific markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns. This study sets grounds for further international collaboration toward the future use of human ES cells in regenerative medicine, drug discovery and toxicology.
Related projects:	Centrum chemické genetiky