Automated confocal live cell microscopy based on spinning disks
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The successful development of non-invasive quantitative visualization techniques for live cell imaging have led to the development of suitable hardware and software for the acquisition and processing of multidimensional image data. Confocal spinning disk systems (based either on a classical Nipkow disk or on the microlens principle) are especially suitable for live cell imaging thanks to high acquisition speed (parallel imaging of thousands of points), high quality image detection (up to 90% quantum efficiency at negligible noise levels with state-of-the-art cameras), low photobleaching and low phototoxicity. Lately, we have been working on the optimization and automation of image acquisition and processing for this type of microscopy. This poster presents full description of our hardware set-up and several applications from live cell imaging that are carried out on this set-up in our laboratory. Special attention is paid to a novel image registration method for the detection and correction of global movement of a cell nucleus in a time-lapse series. The registration method is point-based, precise and efficient. The method can also be used as an initial estimate of matching objects in succesive images in time-lapse series. It has been successfully applied for studying motion of HP1 proteins and telomeres. This research has been supported by the Ministry of Education of the Czech Republic (Projects No. MSM0021622419 and No. LC535). |
| Related projects: |