Gel electrophoresis of metalloproteins and laser ablation with inductively coupled plasma mass spectrometry

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Authors	HAVLIŠ Jan OVADOVÁ Hana VACULOVIČ Tomáš KANICKÝ Viktor
Year of publication	2008
Type	Appeared in Conference without Proceedings
MU Faculty or unit	Faculty of Science
Citation
Description	Optimal laser power density was found between 0.2 GW.cm-2 to 1.5 GW.cm-2. It is characteristic by the lowest RSD and the highest ICP MS signal. ICP MS signal is higher after ablation of gel, which was dried in the drier overnight than after ablation of gel, which was dried between cellophane foil. Background of 59Co signal is higher in the cellophane foil dried gel, but this gel showed easier handling in comparison with gel dried in drier. S/N ratio of LA-ICP-MS gel dried in the gel drier (6.41) is higher than in case of gel dried between cellophane foils (1.91).
Related projects:	Proteins in metabolism and interaction of organisms with the environment Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Molecular basis of cell and tissue regulations Development of new methods for metalloprotein characterisation