The study on the FnrP, NNR and NarR transcription regulator function in bacterium Paracoccus denitrificans at global proteome level
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| Year of publication | 2008 |
| Type | Conference abstract |
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| Description | Paracoccus denitrificans is a non-fermentative, facultatively autotrophic soil bacterium often studied in the field of bioenergetics. In this work, we aimed to analyze the role of three known transcription regulators FnrP, NNR and NarR (with described role as sensors for oxygen and for intermediates of denitrification pathway) in these adaptation processes in P. denitrificans at global proteome level. We analyzed protein composition of four P. denitrificans strains: wild type, FnrP-, NNR- and NarR- mutants grown aerobically, semiaerobically and semiaerobically with nitrate. Total cell lysate proteins were separated using 2-D PAGE, 36 gels in experiment were statistically evaluated and ca. 500 protein spots were analyzed using MALDI-MS/MS and LC-MS/MS. While expected induction of key enzymes involved in denitrification (nitrate reductase, nitrite reductase and N2O reductase) was confirmed, additional proteins were recognized to be modulated by the oxygen and nitrate concentration in growth medium with effects of mutations in fnrP, nnr and narR genes on their expression. Analysis of gene clusters containing up-/down regulated protein genes revealed new interesting relationships between growth conditions, transcription regulators activity and gene expression. These relationships are presented and the range of all three fnr-type transcription regulators action at the global proteome level is discussed. |
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