Role of SRS2 and Mus81/MMS4 in processing recombination/replication intermediates .

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Title in English	ole of SRS2 and Mus81/MMS4 in processing recombination/replication intermediates .
Authors	MATULOVÁ Petra
Year of publication	2008
Type	R&D Presentation
MU Faculty or unit	Faculty of Science
Citation
Description	Homologous recombination (HR) is a process widespread in nature and essential to maintain the integrity of the genome via repair of DNA double-strand breaks (DSBs). Several models were proposed to understand the mechanism of double-strand break repair by HR, identify separate steps and role of the individual proteins involved. The Mus81/MMS4 complex is a DNA structure-specific endonuclease involved in replication fork stability and DNA repair. It seems from genetic experiments that Mus81/MMS4 could interact with Srs2, a helicase which on one hand prevents untimely recombination and on the other hand is necessary for certain pathways of homologous recombination. In our study, we confirmed the interaction between Mus81/MMS4 and Srs2. We then analyzed the stimulation of Mus81/MMS4 nuclease activity by Srs2 and we found out that this stimulation depends on the DNA subtrate involved in the reaction. Our experiments also indicate that the interaction between Mus81/MMS4 and Srs2 is specific.
Related projects:	Proteins in metabolism and interaction of organisms with the environment Biomolecular Structure-function Relationships and their role in the Metabolism