Korelace mezi fenotypovými a molekulárně genetickými metodami identifikace bakterií mléčného kvašení.

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Title in English	Correlation among phenotype and molecular genetic methods of LAB identification
Authors	RITTICH Bohuslav ŠPANOVÁ Alena DRÁB Vladimír HAVLÍKOVÁ Šárka
Year of publication	2008
Type	Article in Periodical
Magazine / Source	Mlékařské listy –Zpravodaj
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Lactobacillus; biochemical tests; PCR; rep-PCR; RAPD; correlation
Description	Bacterial strains of genus Lactobacillus from Czech Colection of Dairy Microorganisms (CCDM) were characterized by polyphasic approache which accounts different genotypic and phenotypic properties. In total 35 Lactobacillus strains were analyzed. Strains were analysed using biochemical tests (API 50CH) and molecular genetic methods: species specific PCR, rep-PCR with (GTG)5 primer and by RAPD with M13 primer. Identity 91.4 % (32 strains from 35 analysed) was found out between biochemical tests and species specific PCR. Strains of species Lactobacillus helveticus were identified mainly. From the results it follows that species specific PCR enabled to obtain most reliable results from molecular biology methods used.
Related projects:	Introduction of new traits for quality and stability improving in variety production of selcted leguminous species by hybridization methods The use of procedures on basis of molecular genetics in acquiring of new bacterial strains with probiotic properties with the aim to increase specific genetic sources for production of functional foods