Comparison of two procedures of loading with voltage-sensitive dye di-4-ANEPPS in rabbit isolated heart
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | Computers in Cardiology |
| MU Faculty or unit | |
| Citation | |
| Web | http://cinc2008.org |
| Field | Physiology |
| Keywords | isolated rabbit heart;voltage-sensitive dye;di-4-ANEPPS |
| Description | Recording of monophasic action potentials (MAPs) by optical method using voltage sensitive dyes has been established as possibility to measure membrane potential changes in certain models. We studied two procedures of isolated rabbit hearts loading with di-4-ANEPPS - "slow" method (perfusion with low concentration of dye for longer time) or "fast" method (single microinjection of highly concentrated bolus of the dye in the aorta). Signal-to-noise ratio was evaluated in each minute of continuous exposure to excitatory light and persistence of dye in the tissue was estimated. We conclude that both methods enable us to record MAPs, but during long exposure to light the persistence of the dye in the tissue is insufficient in "fast" method. Although the "slow" loading affects electrophysiological properties of the heart, in case of rabbit heart it is negligible since its relative resistance to the side-effects of di-4-ANEPPS. |
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