MICROCHIMERISM - LOOK INTO THE INCIPIENT AML RELAPSE?
| Authors | |
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| Year of publication | 2008 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The reoccurrence or increase in autologous hematopoiesis after an allogeneic hematopoietic cell transplantation (allo-HCT) has been linked with incipient leukemia relapse, however, clear definition of such "emergency" in terms of microchimerism has not been defined yet. Aims Since the usual minimal residual disease (MRD) analysis is more sensitive than conventional chimerism analysis and MRD-guided intervention is routinely used after transplantation, here we wanted to explore if more sensitive chimerism analysis (microchimerism) could be used for earlier detection of imminent relapse. Methods We compared conventional polymerase chain reaction (PCR) of minisatellite, microsatellite, or sex specific regions with following fragment analysis (FA) (limit of quantification ~1%), with real-time quantitative PCR (RQ-PCR) of insertion/deletion and sex polymorphism (limit of quantification ~0.1%). |
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