Monitoring of in vitro DNA synthesis and PCR amplification using 7-deaza-purines as electroactive markers

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Authors	FOJTOVÁ Miloslava HORÁKOVÁ Petra PIVOŇKOVÁ Hana ŠIMKOVÁ Eva VYCHODILOVÁ Zdenka HAVRAN Luděk FOJTA Miroslav
Year of publication	2009
Type	Article in Proceedings
Conference	Moderní elektrochemické metody
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	7-deaza purines; electrochemical detection; carbon electrode; repetitive elements
Description	7 deaza purines (particularly 7 deaza guanine) incorporated into the DNA sequence during in vitro extension and/or PCR enable the simple electrochemical detection of the PCR products at the carbon eletrode. Based on the pilot experiments, the utilization of the method in a fast and simple analysis of G rich repetitive sequences in eukaryotic genomes is supposed.
Related projects:	Molecular basis of cell and tissue regulations Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine