Elimination Voltammetry as an Analytical Tool for the DNA sensors
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Proceedings |
| Conference | The 60th Annual Meeting of the International Society of Electrochemistry – book of abstracts |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | DNA sensor; voltammetry |
| Description | The EVLS can be considered as a mathematical model of the transformation of current-potential curves capable of eliminating some selected current components, while conserving others by means of elimination functions. Its application is very simple. The chosen elimination function needs only two or three voltammetric (LSV or CV) curves measured at different scan rates. For totally adsorbed electroactive species the function eliminating charging and kinetic current components, and conserving the diffusion current component, yields for totally adsorbed electroactive species the specific, sensitive and well developed peak-counterpeak (p-cp) signal. This signal, usually 10-20 times higher than corresponding measured voltammetric peak is successfully employed in the analysis of nucleic acids and short homo- or hetero-deoxyoligonucleotides (ODNs) containing adenine and cytosine. Moreover, the EVLS increased the current sensitivity for A and C resolution and, thus, for the recognition of bases sequences in ODN chains. It has been shown that the EVLS in combination with adsorptive stripping procedure is a promising tool for achieving very good resolution of electrode processes, for qualitative and quantitative analysis of ODNs and their components, as well as for identification of ODN structures. |
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