Growth/differentiation factor-15 inhibits differentiation into osteoclasts - A novel factor involved in control of osteoclast differentiation

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Authors	VAŇHARA Petr LINCOVÁ Eva KOZUBÍK Alois JURDIC Pierre SOUČEK Karel ŠMARDA Jan
Year of publication	2009
Type	Article in Periodical
Magazine / Source	Differentiation
MU Faculty or unit	Faculty of Science
Citation
Field	Oncology and hematology
Keywords	GDF-15; osteoclast; LNCaP; RAW264.7; prostate cancer
Description	We show that both pure GDF-15 and the GDF-15-containing growth medium of 1,25(OH)2-vitamin D3-treated prostate adenocarcinoma LNCaP cells suppress formation of mature osteoclasts differentiated from RAW264.7 macrophages and bone-marrow precursors by MCSF/RANKL in dose-dependent manner. GDF-15 inhibits expression of c-Fos and activity of NFkB by delayed degradation of IkB. Moreover, GDF-15 inhibits expression of carbonic anhydrase II and cathepsin K, key osteoclast enzymes, and induces changes in SMAD and p38 signaling.
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