HPLC Measurement of Thiamine and its Phosphorylated Forms in Whole Blood and Plasma: Thiamine Status Assessment

Tomandlová,  Marie; Tomandl,  Josef; Kaňková,  Kateřina

HPLC Measurement of Thiamine and its Phosphorylated Forms in Whole Blood and Plasma: Thiamine Status Assessment

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Authors	TOMANDLOVÁ Marie TOMANDL Josef KAŇKOVÁ Kateřina
Year of publication	2009
Type	Conference abstract
MU Faculty or unit	Faculty of Medicine
Citation
Description	A new gradient ion-paired HPLC utilising fluorescence detection for the simultaneous determination of thiamine and its mono- and diphosphate esters in human whole blood and plasma is developed. The method has been sufficient for the accurate, reliable and reproducible determination of thiamine status in healthy persons an also patients with diabetic nephropathy.
Related projects:	Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie Genetic variability of pentose phosphate pathway as a modulating factor of the onset and progression of diabetic nephropathy