Phosphopeptides analysis using enrichment techniques and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Warning

This publication doesn't include Institute of Computer Science. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	DYČKA Filip LAŠTOVIČKOVÁ Markéta ČERNÝ Martin BRZOBOHATÝ Břetislav BOBÁLOVÁ Janette
Year of publication	2009
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	In the present study, two methods for enrichment of tryptic phosphopeptides were described: IMAC and titanium dioxide chromatography. After enrichment procedure the phosphopeptides were analyzed by MALDI-TOF MS. Alpha-casein was used as model phosphoprotein. The methods were applied to protein mixture of Arabidopsis thaliana.
Related projects:	Proteome dynamics in response to increased cytokinin levels in Arabidopsis