Searching for Genes of Lactococcus lactis subsp lactis Encoding the Bacteriocin Nisin using DNA/DNA Hybridisation

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Authors	DUŠKOVÁ Marta ŠPANOVÁ Alena DRÁB Vladimír RITTICH Bohuslav
Year of publication	2009
Type	Article in Periodical
Magazine / Source	CZECH JOURNAL OF FOOD SCIENCES
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Lactococcus lactis subsp lactis; screening; nisin; gene; PCR probe; DNA/DNA hybridisation
Description	In this work, PCR primers P8 and P9 were used for amplification of a 320 bp long PCR product specific to the nisin gene. The PCR product was labelled with digoxigenine during amplification and used as a DNA probe for the screening of homologous DNA sequences in 7 Lactococcus lactis subsp. lactis strains from the Culture Collection of Dairy Microorganisms (CCDM). Dot blot hybridisation and hybridisation of colonies were used for DNA/DNA hybridisation. It was shown that 6 tested strains of Lactococcus lactis subsp. lactis have genes encoding nisin. One strain had probably a defective gene encoding nisin.
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