Separation of similar yeast strains by IEF techniques
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | Electrophoresis |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | IEF; kandida; biofilm; lýza; identifikace |
| Description | The possibility to separate of the conidia of filamentous fungi, A. niger, A. fumigatus, P. chrysogenum, F. solani and A. flavus, by capillary electromigration techniques was verified in this study. The optimized protocols for their separation by CIEF and CZE with UV and fluorometric detection were developed. According to the preliminary experiments the isoelectric points of the conidia of different strains were not hosts specific. The isoelectric points of the native conidia and the conidia dynamically modified by PB-PEG are comparable. At the electromigration techniques with fluorometric detection down to ten conidia injected into the capillary and dynamically modified by the non-ionogenic tenside were detected |
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