Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | Biochimica et Biophysica Acta - Proteins and Proteomics |
| MU Faculty or unit | |
| Citation | |
| Web | Odkaz na článek |
| Doi | http://dx.doi.org/10.1016/j.bbapap.2010.01.016 |
| Field | Biochemistry |
| Keywords | Paracoccus denitrificans; FNR transcription regulator; Respiratory switch; Two-dimensional gel electrophoresis; Proteome |
| Description | The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three FNR-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this work, we analyzed the protein composition of four strains (wild type, FnrP-, NNR- and NarR-mutant strains) grown aerobically, semiaerobically and semiaerobically in the presence of nitrate to discover the global role of FNR-family transcription regulators using proteomics, with data validation at the transcript and genome levels. Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins were identified using mass spectrometry. The annotated 2-D proteome map provided the most comprehensive coverage of P. denitrificans proteome available to-date and can be accessed on-line at http://www.mpiib-berlin.mpg.de/2D-PAGE/. Our results revealed several new types of regulation. |
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