Spektroskopická a elektroforetická studie reaktivity oxidu osmičelého s thymidinmonofosfátem v přítomnosti dusíkatých ligandů

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Title in English	Spectroscopic and electrophoretic study of OsO4 reactivity with TMP in the presence of nitrogen ligands
Authors	BITTOVÁ Miroslava POLÁŠEK Miroslav KOSTEČKA Pavel MATĚJKA Pavel TRNKOVÁ Libuše
Year of publication	2010
Type	Article in Proceedings
Conference	Sborník 14. česko – slovenské spektroskopické konference
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	osmium tetroxide; thymidine monophosphate; electrophoresis; spectrophotometry; Raman spectroscopy
Description	Study of hybridization and properties of DNA often uses labeling of bases or short nucleotide sequences with fluorescent, radioactive and electrochemical labels. OsO4 complexes with nitrogen containing ligands belong to the last group. They possess selective affinity to pyrimidine bases, especially thymine. Experimental and theoretical results suggest that bidentate N ligands do not react with OsO4 in water solution. The coordination occurs after OsO4 reduction by addition to thymine double bond but the rate of complexation with TMP differs in the dependence of N ligand. During electrophoretical separation we moreover observed 2 products which possibly correspond to two different diastereoisomers
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine