Potential of chloroplast genome in plant breeding

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Authors

ŘEPKOVÁ Jana

Year of publication 2010
Type Article in Periodical
Magazine / Source Czech Journal of Genetics and Plant Breeding
MU Faculty or unit

Faculty of Science

Citation
Web https://doi.org/10.17221/79/2010-CJGPB
Doi http://dx.doi.org/10.17221/79/2010-CJGPB
Field Genetics and molecular biology
Keywords agricultural traits; chloroplast engineering; industrial proteins; molecular pharming; total leaf soluble protein
Description Chloroplast engineering (or chloroplast transformation technology, CTT) is a strategy consisting of inserting a Chloroplast engineering (or chloroplast transformation technology, CTT) is a strategy consisting of inserting a transgene into the chloroplast genome of a plant instead of its nuclear genome. CTT brings advantages such as control of the site of gene insertion, high rates of transgene expression and protein accumulation, lack of transmission of the transgene via pollen due to the fact that plastid genes are maternally inherited and an absence of epigenetic effects. Tobacco remains the species most amenable to CTT to date. Improving agricultural traits is promising application; molecular pharming is another area of chloroplast engineering with high potential. This review summarises the current status of CCT and its potential economic impact from the viewpoint of high levels of transgene expression and high accumulation of foreign proteins.
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