Reversible DNA adsorption on the surfaces of different magnetic nano- and microspheres.

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Authors	ŠPANOVÁ Alena TVRDÍKOVÁ Jana HORÁK Daniel KAMAN Ondřej POLLERT Emil RITTICH Bohuslav
Year of publication	2010
Type	Article in Proceedings
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	magnetic hydrophilic microspheres; P(HEMA-co-GMA); perovskites nanoparticles; PCR-ready DNA; PCR
Description	Non-selective isolation of high-quality DNA can be performed using carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. Analogically the magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation as well as lanthanum manganese perovskites nanoparticles encapsulated into a thin silica shell.
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