Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

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Authors	DIOPAN Václav SHESTIVSKÁ Violetta ZÍTKA Ondřej GALIOVÁ Michaela ADAM Vojtěch KAISER Jozef HORNA Aleš NOVOTNÝ Karel MIROSLAV Liška HAVEL Ladislav ZEHNÁLEK Josef KIZEK René
Year of publication	2010
Type	Article in Periodical
Magazine / Source	Electroanalysis
MU Faculty or unit	Faculty of Science
Citation
Field	Analytic chemistry
Keywords	Thiols; Liquid chromatography; Coulometric detection; Amperometric detection; Heavy metal; Laser induced breakdown spectroscopy
Description	The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy.
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