Gene expression profiling in follicular lymphoma and its implication for clinical practise
| Authors | |
|---|---|
| Year of publication | 2011 |
| Type | Article in Periodical |
| Magazine / Source | LEUKEMIA & LYMPHOMA |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.3109/10428194.2010.531412 |
| Field | Oncology and hematology |
| Keywords | follicular lymphoma; gene expression profiling; lymphomagenesis; prognosis |
| Description | Follicular lymphoma(FL) is characterized by an indolent and relapsing course. Recently, the clinical outcome of FL has been distinguished by immune microenvironment associated gene signatures. In our study, gene expression profiling (GEP) was performed in 31 nonselected patients with follicular lymphoma (FL), 12 of whom were in relapse and the remaining 19 newly diagnosed. A custom oligonucleotide microarray (Agilent 8 x 15K) was used which contained probes for about 3500 genes, including those that had been previously published as demonstrating significant prognostic value. An unsupervised approach was not able to recognize clinically different FLs. As the previously published prognostically relevant gene signatures could not be properly verified, probably due to microarray platform differences, template matching was therefore used in order to define two gene sets with differential gene expression among our samples. These gene sets shared an overrepresentation of genes with similar biological functions and were termed T CELL and PROLIFERATION profiles. The poor profile was then defined by a high PROLIFERATION score (upper tertile) and or low T CELL score (lower tertile). The poor profile cohort contained a significantly higher proportion of relapsed cases (p 0,05 Fishers exact test). Additionally, a comparison of samples from initial diagnosis and from relapse showed significant differences mainly in the T CELL profile (p=0,036 x (2)). This supports the hypothesis that the number of T cells and their expression pattern play a major role in FL development. |
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