Histochemický průkaz těžkých kovů po perorální aplikaci v játrech samic a jejich fétů u laboratorní myši (Mus musculus var. alba)
| Title in English | Histochemical detection of heavy metals after peroral administration in liver of laboratory mice and their foetuses |
|---|---|
| Authors | |
| Year of publication | 2010 |
| Type | Article in Proceedings |
| Conference | Odkaz Ladzianskeho pre budúce generácie |
| MU Faculty or unit | |
| Citation | |
| Field | Morphological specializations and cytology |
| Keywords | Heavy metals; mouse; foetus; liver |
| Description | Distribution of heavy metals and subsequently induced structural changes in some organs of female mice and mouse foetuses after peroral administration of moderate doses of lead, mercury, and cadmium (0.03 mg of metal per mouse and day) was studied in this study. Heavy metals were administered to female mice on days 9 to 20 of pregnancy. There after animals were euthanised by cervical dislocation. Samples of liver of female mice and foetuses were subsequently collected and processed by standard protocol for light and electron microscopy. Histochemical reaction based on metal conversion into appropriate sulphide that conjugates with silver was used for detection of heavy metals. By the light microscopy, deposits of heavy metals, were found in the liver of pregnant mice at the periphery of hepatic lobules in hepatocytes and Kupffer cells, in the liver of foetuses in hepatocytes, endothelial cells of capillaries and erythrocytes. By the electron microscopy, deposits of the reaction product were identified in compartments of cells reacting in the light microscope as follows: in the nuclei and nucleoli, lysosomes, ribosomes of rough endoplasmic reticulum and mitochondria of hepatocytes and Kupffer cells in the liver of females and their foetuses. Structural changes caused by heavy metals were manifested: in the hepatocytes by ruptures of nuclear envelope, obliteration of cisternae of the rough endoplasmic reticulum, separation of ribosomes, edematous mitochondria and destruction of lysosomes. Vacuolization of the cell cytoplasm was also a frequent phenomenon. Damage of hepatocytes was more expressive in the fetal than in the maternal cells. Observed structural changes show that some cells are destroyed by necrosis. High accumulation of metals in organs of pregnant mice and their foetuses was verified in our study. It is concluded that placental barrier does not provide against heavy metal penetration into the foetal organism. Higher accumulation of metals in foetal than in mother liver can be explained by intense metabolism of developing embryonic cells. |
| Related projects: |