Mass Spectrometry Characterization of Plant Phosphoproteins

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Authors	NOVÁKOVÁ Kateřina ŠEDO Ondrej ZDRÁHAL Zbyněk
Year of publication	2011
Type	Article in Periodical
Magazine / Source	CURRENT PROTEIN & PEPTIDE SCIENCE
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://www.ncbi.nlm.nih.gov/pubmed/21348846
Field	Analytic chemistry
Keywords	Plant phosphorylation; phosphoproteomics; mass spectrometry; sample preparation
Description	Protein phosphorylation constitutes a major type of post-translational modifications mobilizing a high number of genes. It is involved in many crucial cell processes and largely participates in the features of the proteome. For several biological and technical reasons, the characterization of protein phosphorylation requires a combination of distinct procedures. In this review, a special emphasis is given to analytical strategies connected with the determination of the presence of phosphorylation sites and their localization by mass spectrometry (MS). The feasibility of selected combinations of analytical approaches for diverse objectives of phosphoproteomic research is discussed.
Related projects:	Molecular basis of cell and tissue regulations Regulation of morphogenesis of plant cells and organs Molecular mechanisms of the cell proliferation and differentiation Development of diagnostics and treatment of serious heart and vascular diseases using genomic and proteomic approaches