Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA Isolated from the Plant Pathogen Agrobacterium tumefaciens C58.

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Authors	HASAN Khomaini FOŘTOVÁ Andrea KOUDELÁKOVÁ Táňa CHALOUPKOVÁ Radka ISHITSUKA M. NAGATA Yuji DAMBORSKÝ Jiří PROKOP Zbyněk
Year of publication	2011
Type	Article in Periodical
Magazine / Source	Applied and Environmental Microbiology
MU Faculty or unit	Faculty of Science
Citation
Doi	http://dx.doi.org/10.1128/AEM.02109-10
Field	Biochemistry
Keywords	Novel Haloalkane Dehalogenase DatA ;Agrobacterium tumefaciens C58
Description	Abstract: We report the biochemical characterization of a novel haloalkane dehalogenase DatA isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilising residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity towards racemic mixtures of chiral brominated alkanes and esters.
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