Bioanalytické studium nádorových markerů karcinomu prostaty na úrovni RNA a proteinu
| Title in English | Bioanalytical study of tumour markers for prostate carcinoma at RNA and protein level |
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| Authors | |
| Year of publication | 2011 |
| Type | Article in Periodical |
| Magazine / Source | Praktický lékař |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | tumour diseases; polymerase chain reaction; electrochemistry; immunodetection; molecularly-biological techniques; tumour markers |
| Description | Current methods for diagnosis of prostate carcinoma do not enable us to distinguish aggressive tumours (significant tumours) from clinically latent tumours (non-significant tumours). This study aims to determine levels of potential clinically important tumour markers such as - alpha-methyl CoA-racemase (AMACR), - caveolin-1, - metallothionein (MT), - p53, - NF-kB, - c-FOS, - c-JUN, - Ki-67, - prostate-specific antigen (PSA), ZIP1 and ZnT-1 in prostatic tissue represented by 22Rv1 (tumour) and PNT1A (healthy) cell lines and in blood serum of patients with histologically evaluated adenocarcinoma (82 tumour patients and 51 healthy volunteers). Based on mRNA expression, it was found that Cav-1, NF-kB, c-FOS and c-JUN were down-regulated and MT,AMACR, PSA, Ki-67, MMP-9 and zinc transporters ZIP1 and ZnT-1 were up-regulated. In serum, the level of MT was significantly enhanced above 1 microM. Caveolin-1 levels were significantly increased in high-grade tumours, which points to the possibility of using this protein as a marker for the aggressive form of prostate carcinoma. |
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