Comparison of Two Electrophoretic Methods Documented by Separation of Dextromethorphan and its Metabolites
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| Year of publication | 2011 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Capillary electrophoresis (CE) is a powerful analytical technique with significant importance in drug discovery because of its unique separation mechanism, speed, efficiency, and versatility. It is well accepted that capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography are still mainly employed for drug analysis. Nevertheless, the uses of other CE modes, non-aqueous capillary electrophoresis (NACE), capillary isotachophoresis, capillary electrochromatography, etc. are increasing and become valuable in this area. In order to distinguish between the use of aqueous and non-aqueous buffers, the term NACE is used to describe the application of pure organic solvents or mixture thereof. Organic solvents are especially suitable for analytes with poor water solubility. CE selectivity can be easily manipulated because of the different degrees of dissociation of the electrolyte, ion-pairing effects, and ion conductivity in organic solvents, because they usually have lower dielectric constants than water. In our study we have focused on the comparison of CZE and NACE conditions. |
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