The effects of PAHs and N-PAHs on retinoid signalling and Oct-4 expression in vitro.

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Authors	BENÍŠEK Martin KUBINCOVÁ Petra BLÁHA Luděk HILSCHEROVÁ Klára
Year of publication	2011
Type	Article in Periodical
Magazine / Source	Toxicology Letters
MU Faculty or unit	Faculty of Science
Citation
Doi	http://dx.doi.org/10.1016/j.toxlet.2010.11.011
Field	Environment influence on health
Keywords	retinoids; differentiation; Oct-4; PAHs; N-PAHs
Description	This study investigated PAHs and N-PAHs interference with retinoid signaling in vitro by modulating all-trans retinoic acid (ATRA) mediated response in a reporter gene assay using P19/A15 cell line. Further, effects on pluripotency and differentiation processes were evaluated by measuring octamer-4 (Oct-4), an important pluripotency marker and master differentiation factor. Our results further extend the knowledge of PAH and N-PAH in vitro effects and indicate that these environmental toxicants may have influence on differentiation process and embryonic development by interfering with ATRA signaling and by modulating levels of Oct-4.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) New molecular biology and biochemical methods to monitor estrogens and other endocrine disruptors in the environment of the C.R. Centrum pro výzkum toxických látek v prostředí