Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA.

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Authors	HUSKA Dalibor ADAM Vojtěch BABULA Petr TRNKOVÁ Libuše HUBÁLEK Jaromír ZEHNÁLEK Josef HAVEL Ladislav KIZEK René
Year of publication	2011
Type	Article in Periodical
Magazine / Source	Microchim. Acta
MU Faculty or unit	Faculty of Science
Citation
Web	https://link.springer.com/article/10.1007/s00604-011-0545-z
Doi	http://dx.doi.org/10.1007/s00604-011-0545-z
Field	Physical chemistry and theoretical chemistry
Keywords	Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles
Description	The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)