Magnetic Hydrophilic Poly(2-Hydroxyethyl Methacrylate-co-Glycidyl Methacrylate) Microspheres for DNA Isolation from Faeces

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Authors	TRACHTOVÁ Štěpánka OBERMAJER Tanja ŠPANOVÁ Alena MATIJAŠIC Bojana Bogovic ROGELJ Irena HORÁK Daniel RITTICH Bohuslav
Year of publication	2012
Type	Article in Periodical
Magazine / Source	Molecular Crystals and Liquid Crystals
MU Faculty or unit	Faculty of Science
Citation
Doi	http://dx.doi.org/10.1080/15421406.2012.635554
Field	Genetics and molecular biology
Keywords	DNA isolation; magnetic microspheres; mouse faeces; P(HEMA-co-GMA); PCR
Description	Magnetic non-porous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA) microspheres containing carboxyl groups were used for DNA isolation from mouse faeces spiked by a probiotic Lactobacillus gasseri K7 strain. The quality of isolated DNA and the presence of target DNA were verified by PCR and real time PCR using primers specific for Lactobacillus genus or primers targeting gassericin A gene of the Lactobacillus gasseri K7 strain. For comparison, two other DNA extraction procedures were used. It was shown that DNA extracted by carboxylcoated P(HEMA-co-GMA) microspheres were sufficient for the amplification of target DNA using PCR and real-time PCR.
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