Estimation of rate constants of drug binding to open channels of cardiac transient outward current
| Authors | |
|---|---|
| Year of publication | 2012 |
| Type | Article in Periodical |
| Magazine / Source | General Physiology and Biophysics |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.4149/gpb_2012_013 |
| Field | Physiology |
| Keywords | Ito channel; Drug binding; Open state; Rate constants; Mathematical model |
| Description | An improved approach to the evaluation of the rate constants of drugs binding to the open channel underlying the transient outward potassium current Ito, is described. It is based on an analysis of a quantitative model formulated by a set of twelve differential equations. The rate constants are calculated from the time constants resulting from an approximation of the time course of apparent inactivation of the recorded Ito by two exponentials in the absence and by three exponentials in the presence, of a blocking agent. The model study confirmed significantly higher accuracy in comparison with the existing electrophysiological method. |
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