Fast and efficient protein enzymatic digestion in proteomics

Warning

This publication doesn't include Institute of Computer Science. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	DYČKA Filip BOBÁĽ Pavel BOBÁLOVÁ Janette
Year of publication	2010
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	In current work we have focused on comparison of the variable performances of the fast enzymatic digestion of proteins using the different techniques: microwave technology, ultrasonic assisted protein enzymatic digestion and proteolysis accelerated by infrared radiation. To demonstrate the suitability of fast procedure, digest bovine serum albumin and in-gel tryptic digestion of proteins extracted from plants (Hordeum vulgare, Arabidopsis thaliana) were employed.
Related projects:	Proteome dynamics in response to increased cytokinin levels in Arabidopsis