SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination

Varování

Publikace nespadá pod Ústav výpočetní techniky, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	BENEŠÍK Martin NOVÁČEK Jiří JANDA Lubomír DOPITOVÁ Radka ŽÍDEK Lukáš DOŠKAŘ Jiří RŮŽIČKOVÁ Vladislava MOŠA Marek PANTŮČEK Roman
Rok publikování	2012
Druh	Konferenční abstrakty
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
Popis	Staphylococcus aureus is a major causative agent of human and animal diseases. The increasing number of pathogenic strains resistant to antimicrobial drugs is a serious public health problem that can be solved by applications of phage therapy as a suitable alternative to antibiotics treatment. Currently the research focus on the phage encoded antimicrobial proteins applicable to combat bacterial infections, such as phage endolysins that digest the cell wall of bacteria. In this study we focused on endolysin of staphylococcal bacteriophage 812, a member of SPO1-like viruses from family Myoviridae. Preliminary results of the NMR analysis confirmed the presence of the well defined structure of the studied construct, and provided the sequential assignment of all resonance frequencies along the peptide backbone allowing us to assess the presence of the secondary structure motifs.
Související projekty:	Výzkum stafylokokových bakteriofágových mutant s širokým spektrem hostitelů