Apolipoprotein E gene polymorphisms in chronic periodontitis
| Autoři | |
|---|---|
| Rok publikování | 2013 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Objective: Periodontal diseases can be related to common systemic conditions, and, as recently described, alterations in blood lipid levels. The aim of this study was to analyze two apolipoprotein E (ApoE) gene polymorphisms in patients with chronic periodontitis (CP) in relation to periodontopathic bacteria and lipid levels. Methods: 469 unrelated subjects were included in this case-control study. Genomic DNA of 294 patients with CP and 175 healthy/non-periodontitis controls were genotyped, using the real-time polymerase chain reaction (RT-PCR) method monitored by SYBR Green, for ApoE (rs429358 and rs7412) gene polymorphisms. Subgingival bacterial colonization was investigated by the DNA-microarray based on a periodontal pathogen detection kit and lipid levels were measured in a subgroup of subjects. Results: There was no evidence for a significant association between ApoE gene polymorphisms and CP (E2/E2 vs. E3/E3: OR=0.63, 95%CI=0.12-3.15; E2/E3 vs. E3/E3: OR=1.61, 95%CI=0.84-3.11; E2/E4 vs. E3/E3: OR=1.25, 95%CI=0.23-6.95; E3/E4 vs. E3/E3: OR=1.11, 95%CI=0.65-1.88; E4/E4 vs. E3/E3: OR=0.63, 95%CI=0.15-2.55; allele E2 vs. allele E3: OR=1.26, 95%CI=0.74-2.14; allele E4 vs. allele E3: OR=0.97, 95%CI=0.63-1.51). Patients with CP had increased total cholesterol and LDL levels compared to controls (P<0.05), however no significant differences were found for triglyceride and HDL levels. Gene variability in the ApoE gene influenced LDL levels marginally (P=0.07) but did not modify total cholesterol, triglyceride and HDL levels or the occurrence of periodontal pathogens in subgingival pockets. Conclusions: In Czech population studied, ApoE genetic variations rs429358 and rs7412 were not associated with susceptibility to and severity of chronic periodontitis or with the presence of periodontopathic bacteria. |
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