Improved IgH-based MRD Detection By Using Droplet Digital PCR: a Comparison With Real Time Quantitative PCR in MCL and MM

• Autoři: DRANDI Daniela; BEŠŠE Lenka; DANI Nadia; FERRERO Simone; MONITILLO Luigia; MANTOAN Barbara; GENUARDI Elisa; BARBERO Daniela; GAMBELLA Manuela; BARBERIO Davide; GHIONE Paola; GUARONA Guglielmo; SARACI Elona; OMEDE Paola; HÁJEK Roman; CORTELAZZO Sergio; PALUMBO Antonio; BOCCADORO Mario; INGHIRAMI Giorgio; LADETTO Marco
• Rok publikování: 2013
• Druh: Konferenční abstrakty
• Fakulta / Pracoviště MU: Lékařská fakulta
• Popis: Minimal residual disease (MRD) detection is a well defined prognostic tool for response evaluation in hematological malignancies. Real time quantitative PCR (RQ-PCR) has become the settled and standardized method for MRD assessment in lymphoid disorders (van der Velden et al., Leukemia 2007). However, RQ-PCR based MRD detection relies on a relative quantification approach, since it requires a reference standard curve and an appropriate housekeeping gene for the normalization. Droplet Digital PCR (DD-PCR), applying the principle of limiting dilution of DNA and single molecule detection, allows reliable absolute tumor burden quantification at molecular level. In this study we performed a comparison of immunoglobulin heavy chain gene based MRD detection (IgH-MRD) performed by RQ-PCR and DD-PCR, to assess whether DD-PCR could overcome some RQ-PCR limitation and achieve superior sensitivity, robustness and accuracy.

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