In-Cell NMR and EPR Spectroscopy of Biomacromolecules
| Autoři | |
|---|---|
| Rok publikování | 2014 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Angewandte Chemie International Edition |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://onlinelibrary.wiley.com/doi/10.1002/anie.201311320/abstract |
| Doi | http://dx.doi.org/10.1002/anie.201311320 |
| Obor | Biochemie |
| Klíčová slova | MAGNETIC-RESONANCE-SPECTROSCOPY; XENOPUS-LAEVIS OOCYTES; ELECTRON-PARAMAGNETIC-RESONANCE; PROTEIN-PROTEIN INTERACTIONS; HUMAN TELOMERIC DNA; DISTANCE MEASUREMENTS; ESCHERICHIA-COLI; LIVING CELLS; F-19 NMR; PHYSIOLOGICAL CONDITIONS |
| Popis | The dream of cell biologists is to be able to watch biological macromolecules perform their duties in the intracellular environment of live cells. Ideally, the observation of both the location and the conformation of these macromolecules with biophysical techniques is desired. The development of many fluorescence techniques, including super-resolution fluorescence microscopy, has significantly enhanced our ability to spot proteins and other molecules in the crowded cellular environment. However, the observation of their structure and conformational changes while they attend their business is still very challenging. In principle, NMR and EPR spectroscopy can be used to investigate the conformation and dynamics of biological macromolecules in living cells. The development of in-cell magnetic resonance techniques has demonstrated the feasibility of this approach. Herein we review the different techniques with a focus on liquid-state in-cell NMR spectroscopy, provide an overview of applications, and discuss the challenges that lie ahead. |
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