Adsorption of cisplatin on plasma trated sufaces: a mass spectrometric study
| Autoři | |
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| Rok publikování | 2012 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Cisplatin (Fig. 1) is widely used as anti tumor drug. It shows several side effects, in particular neuro and nephro toxicity [1,2]. It affects the DNA, RNA, or protein synthesis. Therefore, cisplatin was classified by the International Agency for Research on Cancer (IARC) as carcinogenic, mutagenic, and teratogenic compound for humans. Potential health risk for people arises from direct contact, contamination of the work area or of packaging material [3]. For this reason, side effects due to occupational exposure to cisplatin have been reported with increasing frequency [4]. Fig. 1: Chemical structure of cisplatin. It has been shown that drug vials may already be contaminated with cisplatin when delivered from the manufacturer [5]. In addition, theoretical studies have shown that cisplatin may interact with hydrated silica [6]. The surface properties and the chemical composition of the materials used to store and handle cisplatin influence its adsorption. The activation and/or functionalization of surfaces may enhance or suppress the drug adsorption. Treatment of glass surfaces with non-thermal plasma [7,8] allows to obtain unique modifications with respect to those obtained by conventional chemical methods [9]. For example, properties like hydrophilicity, adhesivity and wettability are altered. In this work the adsorption of cisplatin on i) glass or ii) silicon wafer before and after treat ment with non-thermal plasma was evaluated by laser desorption ionization mass spectrometry (LDI MS). 2 Results and discussion Non-thermal plasma was obtained by diffuse coplanar surface barrier discharge (DCSBD) (Fig. 2). Fig. 2: Device used for non-thermal plasma treatment. Silicon wafers and glass samples were cleaned in isopropyl and cyclohexan (50:50) and dried by a stream of argon. After, they were treated with plasma for 30 seconds and then immersed for 20 minutes in a commercial solution of cisplatin (PLATIDIAM® inj. 10) from Lachema (Brno, Czech Republic). The samples were dried again and stored for the mass spectrometric analysis. Mass spectra were recorded in positive ion mode on AXIMA Resonance mass spectrometer from Shimadzu (Manchester, UK). The instrument is equipped with a quadrupole ion trap (QIT) and a reflectron time of flight (R TOF) mass analyzer. It was found that the adsorption of cisplatin on the glass surface is lower after plasma treatment. An example of mass spectra is given in Fig. 3 (spectra are normalized). Fig. 3: Mass spectra of PtH+ species detected on a) untreated and b) plasma treated glass. It was found that cisplatin is not adsorbed on the surface of silicon wafers either before or after plasma treatment. 3 Conclusions Plasma treatment of glass was found suitable to significantly reduce the adsorption of cisplatin on the surface. Silicon wafers either untreated or plasma treated adsorb cisplatin in negligible amount. |
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