The Kjeldahl Method as a Primary Reference Procedure for Total Protein in Certified Reference Materials Used in Clinical Chemistry. I. A Review of Kjeldahl Methods Adopted by Laboratory Medicine
| Autoři | |
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| Rok publikování | 2015 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Critical Reviews in Analytical Chemistry |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://www.tandfonline.com/doi/abs/10.1080/10408347.2014.892820#.VPRShSwkUXk |
| Doi | http://dx.doi.org/10.1080/10408347.2014.892820 |
| Obor | Analytická chemie, separace |
| Klíčová slova | calibration; reference materials; Kjeldahl methods; clinical chemistry; total protein |
| Popis | We found previously that albumin-calibrated total protein in certified reference materials causes unacceptable positive bias in analysis of human sera. The simplest way to cure this defect is the use of human-based serum/plasma standards calibrated by the Kjeldahl method. Such standards, commutative with serum samples, will compensate for bias caused by lipids and bilirubin in most human sera. To find a suitable primary reference procedure for total protein in reference materials, we reviewed Kjeldahl methods adopted by laboratory medicine. We found two methods recommended for total protein in human samples: an indirect analysis based on total Kjeldahl nitrogen corrected for its nonprotein nitrogen and a direct analysis made on isolated protein precipitates. The methods found will be assessed in a subsequent article. |
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